IQ-TREE is an efficient software for constructing phylogenetic trees, renowned for its speed, accuracy, and rich functionality. It employs the maximum likelihood method and uses an innovative model selection mechanism (ModelFinder) to automatically determine the optimal nucleotide or amino acid substitution model. It also utilizes the ultra-fast bootstrap method (UFBoot2) to evaluate the reliability of tree nodes. IQ-TREE can handle large-scale datasets (thousands of sequences) and its computational efficiency is significantly superior to traditional maximum likelihood software (such as RAxML), making it a popular choice for modern phylogenetic analysis.