Protein-protein interaction assays, transient gene transformation and exogenous hormone treatment experiments were used to characterize the cascade transcriptional regulatory pathway.
Core Findings
Identified the complete negative regulatory cascade VvPUB8-VvbHLH93-VvMYB15/VvMYB5a
Confirmed that exogenous methyl jasmonate suppresses anthocyanin synthesis in grape peel via this ubiquitination regulatory module
Provided theoretical support for precise hormone regulation and directional quality improvement during grape fruit coloration
Publication Note: Manuscript titled The VvPUB8–VvbHLH93–VvMYB15/VvMYB5a module inhibits the synthesis of anthocyanins in grape in response to MeJA accepted by Journal of Integrative Plant Biology (IF = 12.5); official DOI pending journal finalization.