Long-read sequencing combined with Hi-C technology was adopted to construct phased genomes; transcriptome and metabolome joint analysis was performed to dissect cell wall metabolic pathways during fruit development.
Key Findings
Four sets of homologous chromosome haplotype genomes of tetraploid Chinese cherry were successfully separated, with assembly completeness reaching 98.60%.
Haplotype-specific differential expression of genes in cell wall pectin degradation and lignin synthesis pathways was clarified as the core driver of fruit softening.
Molecular markers available for improving fruit firmness and extending shelf life were screened to support directional breeding for superior cherry fruit quality.
Related paper titled Haplotype-resolved genome assembly for tetraploid Chinese cherry (Prunus pseudocerasus) offers insights into fruit firmness was published in Horticulture Research (Impact Factor = 7.3).