Flow cytometry was used to determine C-values of genomes, and chloroplast molecular markers were adopted for phylogenetic analysis to dissect evolutionary trajectories of wild sugarcane germplasm.
Key Findings:
Genome sizes of multiple wild germplasms in the Saccharum complex were precisely quantified, and the variation in genome expansion among species was assessed.
Phylogenetic branching relationships of wild relatives in Saccharum were clarified, resolving some controversies in traditional taxonomy.
The results provide evolutionary evidence for parental selection in sugarcane crossbreeding and germplasm evaluation.