PacBio HiFi sequencing combined with Hi-C was utilized for chromosomal anchoring. Population differentiation analysis was performed to screen selective sweep loci related to domestication.
Key Findings
The ancient whole-genome duplication event shared by Campanulales was clarified, and chromosomal rearrangement patterns during independent evolutionary processes of Codonopsis pilosula were identified.
Expanded gene families critical for biosynthetic pathways of codonopsis polysaccharides and triterpenoid saponins were screened.
Selected gene loci associated with quality and stress resistance traits during artificial domestication were mapped, providing molecular basis for selective breeding of authentic Codonopsis pilosula cultivars and directional improvement of medicinal material quality.
Full paper title: Chromosome-scale genome assembly of Codonopsis pilosula and comparative genomic analyses shed light on its genome evolution, published in Frontiers in Plant Science (IF = 5.87).