PacBio sequencing combined with Hi-C technology was applied to construct chromosome-scale genome assembly. Transcriptomic differences between normal liver and fatty liver samples were compared to screen susceptibility-related genes.
Key Findings:
A high-quality chromosome-level reference genome of Muscovy duck with good contiguity was obtained.
Differential genes related to lipid deposition and inflammation regulation were identified, revealing unique genomic features underlying the tolerance to fatty liver injury in Muscovy duck.
This genome resource supports waterfowl breeding targeting fatty liver metabolism and further research on avian lipid metabolism.