Multi-platform sequencing was applied for genome assembly; multi-omics approaches were combined to screen cold-resistant genes followed by functional validation.
Key Findings
A complete chromosome-level genome of Prunus tomentosa was obtained, and the phylogenetic framework of Prunus species was reconstructed.
PtIMP3 and PtMIOX1L were identified to positively mediate plant cold resistance by regulating inositol accumulation.
Valuable genetic resources were provided for cold-hardy cherry rootstock breeding and stress-resistance molecular improvement of Prunus fruit trees.
The corresponding article New insights into genome assembly at the chromosome-level of Prunus tomentosa in evolution and cold tolerance was published in iMeta (IF: 23.8).