Third-generation long-read sequencing integrated with Hi-C enabled chromosomal anchoring. Transcriptomic data was combined to refine gene model annotation, and comparative genomics analysis was carried out among closely related species.
Key Findings
A complete genome comprising 13 chromosomes was assembled, with over 30,000 protein-coding genes accurately annotated.
Expanded enzyme gene families governing biosynthesis of characteristic lignans and medicinal alkaloids in Flueggea virosa were localized.
Rules of chromosomal evolutionary rearrangement across Phyllanthaceae species were clarified, offering reliable genomic data support for medicinal ingredient development and germplasm resource evaluation of Flueggea virosa.
Full paper title: Chromosome-level genome assembly and annotation of Flueggea virosa (Phyllanthaceae), published in Scientific Data (IF = 8.9).