Long-read sequencing combined with Hi-C technology was applied to construct a haplotype-resolved genome. Transcriptomics and metabolomics were jointly utilized to dissect pathways governing resin biosynthesis and drought stress response.
Key Findings
Two haplotypes of homologous chromosomes were successfully separated with excellent assembly completeness, clarifying structural variants and divergent gene expression between haplotypes.
Critical candidate gene clusters responsible for biosynthesis of flavonoid active substances such as dracaenin were identified.
Molecular evolutionary mechanisms of wound-induced resin production and extreme drought adaptation in Dracaena were elucidated, providing genomic evidence for resource conservation and standardized artificial cultivation of Dracaena cambodiana.
Full paper title: Chromosome-level and haplotype-resolved genome assembly of Dracaena cambodiana (Asparagaceae), published in Scientific Data (IF = 8.9).