PacBio long-read sequencing and Hi-C scaffolding were integrated for chromosomal anchoring; systematic functional annotation was conducted on key genes involved in polysaccharide synthesis and photosynthetic metabolism.
Key Findings
A complete genome covering 46 chromosomes was assembled, with a total genome length of 2.37 Gb and a chromosomal anchoring rate of 99.83%.
The amplification pattern of key enzyme gene families for paramylon synthesis was resolved, revealing the genetic basis underlying efficient accumulation of energy storage polysaccharides in E. gracilis.
Elite genes related to stress tolerance and efficient photosynthesis of Euglena were excavated, providing editing targets for engineered high-yield E. gracilis strains.
Related paper titled A chromosome-level genome assembly for the paramylon-producing microalga Euglena gracilis was published in Scientific Data (Impact Factor = 8.9).
Research Progress
A chromosome-level high-quality genome assembly of paramylon-producing microalga Euglena gracilis
👤 Zixi Chen, Yang Dong, Shengchang Duan
📅 七月 16, 2024
📍 Scientific Data
👁 Views 1
Summary:Multi-platform sequencing was utilized to construct a chromosome-level reference genome of Euglena gracilis; the evolutionary pattern and regulatory network of gene families related to paramylon synthesis were analyzed, laying a genomic foundation for resource-oriented development of microalgae.
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External resource
https://www.nature.com/articles/s41597-024-03287-9 →